Kaoutar El Handi, Marwa Mourou, Khaoula Habbadi, El Hassan Achbani, Miloud Sabri, Majida Hafidi, Maroun El Moujabber, Franco Valentini
Abstract
Pure culture isolation of the Xylella fastidiosa strain related to the olive quick decline
syndrome (OQDS), lately noticed in Apulia (southern Italy) was attempted from
naturally infected symptomatic plants mainly Olea europaea L., Polygala myrtifolia and
Rosmarinus officinalis using printing and sap extraction methods. Prior to isolation, RTLAMP and qPCR were used to determine the presence of Xylella fastidiosa in all hosts.
Pure bacterial cultures were obtained from both Olea europaea L. and Polygala
myrtifolia extracts plated in buffered cysteine-yeast extract (BCYE) media. Two olive
isolates were subsequently typed using the Multilocus Sequencing System (MLST).
Findings indicate that beside Olea europaea, Polygala myrtifolia seems to have a good
potential for isolation, and printing method has presented significantly better results.
MLST shows genetic commonality with De Donno (ST53) strain and ascertain that
Apulia region still have the same sequence type ST53 in the region.
Keywords : Xylella fastidiosa; OQDS; RT-LAMP; qPCR; Pure culture isolation; BCYE;
Printing method; sap extraction method; MLST.
